Journal: Journal of Cellular and Molecular Medicine

Article Title: Escitalopram oxalate induces apoptosis in U‐87 MG cells and autophagy in GBM 8401 cells

doi: 10.1111/jcmm.13372

Figure Lengend Snippet: Representative results of flow cytometry in GBM 8401, U‐87 MG and Hs683 cells in the presence of escitalopram oxalate for 24 hrs. Arrow indicated the sub‐G1 proportion. Similar results were observed in three repeated experiments.

Article Snippet: Human brain astrocyte cells (HBA) and human malignant gliomas cell lines, GBM8401, U‐87MG and Hs683, were purchased from ScienCell (CA, USA) and ATCC (Rockville, MD, USA), respectively.

Techniques: Flow Cytometry

Journal: Signal Transduction and Targeted Therapy

Article Title: Identification of the receptor of oncolytic virus M1 as a therapeutic predictor for multiple solid tumors

doi: 10.1038/s41392-022-00921-3

Figure Lengend Snippet: MXRA8 promotes the therapeutic efficacy of OVM in vitro and in vivo. a The infection rates of OVM-GFP in control, ΔMXRA8 (sgRNA-1) and ΔMXRA8 (sgRNA-1)+MXRA8 Hs578T or HepG2 cells (MOI of 0.1 for 48 h at 37 °C) by flow cytometry (three experiments, n = 9; one-way ANOVA; mean ± s.d.). b, c MXRA8-overexpressing cells and control cells were infected with OVM-GFP virus at a multiplicity of infection (MOI) of 1 (HeLa cells, 48 h; HT29 cells, 24 h), after which the phase-contrast and fluorescence microscopy images were captured, and further processed for detection of GFP expression by flow cytometry (three experiments, n = 9; one-way ANOVA; mean ± s.d.). Scale bars: 50 μm. d The viral titer of tumor cells that treated with OVM at 0.01 MOIs for 72 h by CCID50 assay (three experiments, n = 9; one-way ANOVA; mean ± s.d.). e The viability of tumor cells treated with OVM at the respective MOIs for 72 h by MTT assay (three experiments, n = 9; one-way ANOVA; mean ± s.d.). f Timeline of the experimental arrangement for g – o . g Tumor growth curves of the average HT29 tumor volumes in each group. h Kaplan–Meier survival curves of HT29 tumor-bearing mice by log-rank test. i The amount of OVM in tumor tissues was measured by qPCR at 2 days after the 10th injection ( n = 4, per group). j Tumor growth curves of the average HeLa tumor volumes in each group. k HeLa tumor weights on the 27th day after the first OVM treatment (one-way ANOVA; mean ± s.d.). l Photograph of HeLa tumor tissues on the 27th day. m Tumor growth curves of the average HepG2 tumor volumes in each group. n HepG2 tumor weights on the 23rd day after the first OVM treatment (one-way ANOVA; mean ± s.d.). o Photograph of HepG2 tumor tissues on the 23rd day. n.d., not detectable. Tumor growth curves were analyzed by repeated-measures ANOVA in a general linear model. * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: Cell lines were purchased from ATCC (HepG2, LoVo, U-87MG, and Vero cell lines), Guangzhou Cellcook Biotech (HeLa, HUVEC and BHK-21 cell lines), Guangzhou Jennio Biotech (Hs578T cell line), and Shanghai Institute of Cell Biology (769-P and HT29 cell lines).

Techniques: Drug discovery, In Vitro, In Vivo, Infection, Control, Flow Cytometry, Virus, Fluorescence, Microscopy, Expressing, MTT Assay, Injection

Journal: Signal Transduction and Targeted Therapy

Article Title: Identification of the receptor of oncolytic virus M1 as a therapeutic predictor for multiple solid tumors

doi: 10.1038/s41392-022-00921-3

Figure Lengend Snippet: The tumor selectivity of OVM is modulated by a comprehensive effect of MXRA8 and ZAP. a Spearman’s correlation coefficient of cell viability under OVM treatment and ZAP expression levels. R indicates the Spearman’s correlation coefficient. mRNA expression levels of ZAP in tumor cell lines were retrieved from the CCLE database. b The protein expression levels of ZAP in HepG2, Hs578T, HeLa, and HT29 cells. c The infection rates of OVM-GFP in sgCtrl, sgCtrl + ZAP, ΔMXRA8 + ZAP Hs578T and HepG2 cells (MOI of 0.1) by flow cytometry (three experiments, n = 9; one-way ANOVA; mean ± s.d.). d, e The infection rates of HeLa cells transfected with siZAP or siNC and incubated with OVM-GFP virus (three experiments, n = 3; one-way ANOVA; mean ± s.d.). The protein expression levels of ZAP, NS3, and E1 were measured by Western blot. f The cell viability of HeLa tumor cells treated with OVM at the respective MOIs for 72 h was measured by MTT assay (three experiments, n = 9; one-way ANOVA; mean ± s.d.). * P < 0.05; ** P < 0.01; *** P < 0.001

Article Snippet: Cell lines were purchased from ATCC (HepG2, LoVo, U-87MG, and Vero cell lines), Guangzhou Cellcook Biotech (HeLa, HUVEC and BHK-21 cell lines), Guangzhou Jennio Biotech (Hs578T cell line), and Shanghai Institute of Cell Biology (769-P and HT29 cell lines).

Techniques: Expressing, Infection, Flow Cytometry, Transfection, Incubation, Virus, Western Blot, MTT Assay